| Wheat germ (Triticum aestivum) calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26). Wheat germ calmodulin coupled primarily through SH group (Cys26) to epoxy activated resin. Coupled at 5 mg per mL and useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles. Loading conditions: 100 µM Ca2+, with protease inhibitors and appropriate buffers. Elution conditions: Buffers with 1 mM EDTA or EGTA. | |
| Reference Price: | $250.00 Click to Order |
| More Information: | CaMTag™ HD Capture - Cancer Research - Calcium signaling - Strasburg, G.M., et al. Site-specific derivatives of wheat germ calmodulin. Interactions with troponin and sarcoplasmic reticulum. J Biol Chem 1988 263:12161. |
| Wheat germ (Triticum aestivum) calmodulin (CaM) sequence is unique in that it contains cysteine at residue position 26 (Cys26). Wheat germ calmodulin coupled primarily through SH group (Cys26) to epoxy activated resin. Coupled at 5 mg per mL and useful for pull down experiments and proteomic applications requiring a high capacity precipitation reagent. Dense resin can be easily separated from extracts by centrifugation using batch or spin column procedures. Resin can be reused but proteolysis from crude samples will reduce binding capacity on repeat cycles. Loading conditions: 100 µM Ca2+, with protease inhibitors and appropriate buffers. Elution conditions: Buffers with 1 mM EDTA or EGTA. | |
| Reference Price: | $250.00 Click to Order |
| More Information: | CaMTag™ HD Capture - Cancer Research - Protein Kinases - Strasburg, G.M., et al. Site-specific derivatives of wheat germ calmodulin. Interactions with troponin and sarcoplasmic reticulum. J Biol Chem 1988 263:12161. |
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